Twenty autologous umbilical cord blood from private cord blood banks were evaluated for thaw characteristics: "only 11% of evaluable autologous umbilical cord blood achieved the minimum total nucleated cell count of at least 9.0×10(8) to meet the National Cord Blood Inventory banking threshold and only 50% met the minimum of 5.0×10(8) TNC count for Food and Drug Administration cord blood licensure eligibility."
研究對私人臍帶血庫的21個自體臍血進行解凍後的特點評估“只有11％的自體臍血達到了9.0×10(8)的最低總核細胞數目, 以滿足國家臍帶血庫存的門檻; 此外, 亦只有50％的自體臍血達到了5.0×10(8)總核細胞數目,以滿足食品和藥物管理局臍帶血執照的資格.
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Transfusion. 2012 Feb 10. doi: 10.1111/j.1537-2995.2011.03556.x. [Epub ahead of print]
Characteristics of thawed autologous umbilical cord blood.
Rosenau EH, Sugrue MW, Haller M, Fisk D, Kelly SS, Chang M, Hou W, Eldjerou L, Slayton W, Cogle CR, Wingard JR.
Source
From the Department of Medicine, the Department of Pediatrics, and the Department of Epidemiology and Health Policy Research, University of Florida, and Shands Hospital at the University of Florida, Gainesville, Florida; and the Department of Pediatrics Patient Care, University of Texas MD Anderson Cancer Center, Houston, Texas.
Abstract
BACKGROUND:
Autologous umbilical cord blood (AutoUCB) has historically been cryopreserved for potential use in hematopoietic transplantation. Increasingly, private AutoUCB banking is performed for therapies unavailable today. A Phase I trial using AutoUCB treatment for early pediatric Type 1 diabetes afforded us an opportunity to analyze characteristics of AutoUCBs.
STUDY DESIGN AND METHODS:
Twenty AutoUCBs from AABB-accredited private cord blood banks (CBBs) were evaluated for collection, processing, cryopreservation, and thaw characteristics. Using a standardized thaw-wash method, AutoUCBs were assessed for viable total nucleated cells (vTNCs), viable CD34+ (vCD34+), and colony-forming unit-granulocyte-macrophage counts. Postthaw %vTNC recoveries were compared against processing characteristics and analyzed according to processing method, cryopreservation volume, concentration, container, and length of storage.
RESULTS:
AutoUCB collection volumes (19.9-170 mL), cryopreserved TNC counts (7.6× 10(7) -3.34×10(9) ), %TNC processing recoveries (39%-100%), postthaw %vTNC recoveries (58%-100%), and %vCD34+ recoveries (26%-96%) varied widely. Regarding cell dose requirements, only 11% of evaluable AutoUCBs achieved the minimum TNC count of at least 9.0×10(8) to meet the National Cord Blood Inventory banking threshold, and only 50% met the minimum of 5.0×10(8) TNC count for Food and Drug Administration cord blood licensure eligibility. %vTNC recoveries correlated with %vCD34+ recoveries (R=0.7; p=0.03). Length of storage, cryopreservation volume, concentration, and container type did not affect postthaw %vTNC recoveries. CBB processing method, however, was associated with %vTNC postprocessing recoveries, with unmanipulated and plasma-depleted AutoUCBs having the highest postthaw %vTNC recovery, followed by RBC-depleted and density gradient-separated AutoUCBs.
CONCLUSION:
The high variability and low counts found in AutoUCB banking suggest that further standardization of characterization, collection, and processing procedures is needed.
© 2012 American Association of Blood Banks.
- PMID:
- 22321210
- [PubMed - as supplied by publisher]
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