Protein Injection Strategy

(Continuously Updating)

Paper 1: Wnt7a treatment ameliorates muscular dystrophy

Strategy :

1.  Wnt7a treatment efficiently induced satellite cell expansion and myofiber hypertrophy in treated mucles in mdx mice.
1. Wnt7a signaling through its receptor Fzd7 accelerates and augments regeneration by:
1. stimulating satellite stem cell expansion through the planar cell polarity pathway, as well as,
2. myofiber hypertrophy through the AKT/mammalian target of rapamycin (mTOR) anabolic pathway. 

Application:

1. Duchenne muscular dystrophy (DMD)

References: 

1. J. von Maltzahn, J.-M. Renaud, G. Parise, M. A. Rudnicki.Wnt7a treatment ameliorates muscular dystrophy.Proceedings of the National Academy of Sciences, 2012; DOI: 10.1073/pnas.1215765109

Cells Rejuvenation Strategy

(Continuously Updating)

Paper 1: Aged human cells rejuvenated by cytokine enhancement of biomaterials for surgical ventricular restoration.

Strategy : 

1.  cytokine enhancement of a biodegradable patch
1.  covalently immobilized 2 proangiogenic cytokines (vascular endothelial growth factor and basic fibroblast growth factor) onto porous collagen scaffolds.
2.  seeded human mesenchymal stromal cells from old donors into the scaffolds with  growth factors. 

Application: 

1. Surgical ventricular restoration (SVR)

References:

1. Kang K, Sun L, Xiao Y, Li SH, Wu J, Guo J, Jiang SL, Yang L, Yau TM, Weisel RD, Radisic M, Li RK. Aged human cells rejuvenated by cytokine enhancement of biomaterials for surgical ventricular restoration. J Am Coll Cardiol. 2012 Nov 20;60(21):2237-49. doi: 10.1016/j.jacc.2012.08.985. (Department of Cardiovascular Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin, China; Division of Cardiovascular Surgery and Toronto General Research Institute, University Health Network and Department of Surgery, Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, Canada.) PMID: 23153846